MiniMUGA Sample Report - Initial Version
MMRRC Strain Name B6;129S7-Amhr2tm3(cre)Bhr/Mmnc
MMRRC Strain ID 14245
Sample ID ZX0193
Genotyping Quality Excellent (6 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XX
2023-07-07T14:34:16.828882 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
C57BL/6J Homozygous 2774 100.0% 99.9%
C57BL/6J X 129S6/SvEvTac Heterozygous 1 0.0% 0.1%
Total 2775 100.0% 100.0%
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - + - - - - - - - + - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6J, C57BL/6JRj 127 2 132 97.7%
C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 21 0 21 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JRj 5 0 5 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 2 0 2 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 1 0 1 100.0%
C57BL/6J 1 0 3 33.3%
C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 1 0 2 50.0%
C57BL/6J, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 1 0 1 100.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: C57BL/6J
    • C57BL/6J: 161 / 167 (96.4%)
Strain Reproducibility Strain Reproducibility Samples: ZX0193, HT0195
Inbreeding Level: 99.7%
Y Chromosome: N/A
Congenicity Level: 99.8%
Donor Contribution in Linked Interval: 2.0Mb

The genome of this strain is not reproducible because there are regions that are still segregating (at least 0.3%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown.
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two XX samples (HT0195 and ZX0193) at the time of importation and the conclusions are consistent in all samples.. The results of the analysis are congruent with the SDS and ZX0193 is the representative sample selected for the report. The primary (host) background is C57BL/6J. The secondary (donor) background is consistent with the 129S strain group. A 129S7SvEv reference is not available in miniMUGA and is the donor ES cell background indicated in the SDS. The mitochondrial genome is consistent with at least one backcross to a female from the host background. The background of the Y chromosome was not assessed because both samples genotyped were XX. The region containing the allele of interest has the expected donor background (129S). Both sample(s) are heterozyous for the donor background at the locus. The following constructs are detected in both samples: Cre, IRES.

The genome of the MMRRC: 14245 mice strain is 99.7 % inbred and 100% congenic at the strain level. 0.3% of the genome is segregating for the secondary (donor) background.
WARNINGS
  • This sample likely has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect. This can potentially be addressed with input from the user.
Diplotype Intervals
Report generated on 2023-07-07 14:34