MiniMUGA Sample Report - Initial Version
MMRRC Strain Name B6.Cg-Get3tm1Hbha/Mmnc
MMRRC Strain ID 10859
Sample ID VM8959
Genotyping Quality Excellent (4 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2023-04-11T18:39:47.994628 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
C57BL/6J and C57BL/6NTac Homozygous 1755 66.7% 65.3%
129X1/SvJ Homozygous 143 5.4% 6.7%
C57BL/6J and C57BL/6NTac X 129X1/SvJ Heterozygous 692 26.3% 27.2%
Unexplained Homozygous 42 1.6% 0.8%
Total 2632 100.0% 100.0%
* Please see substrain reproducibility section
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - - - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6J, C57BL/6JRj 44 62 139 76.3%
C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 4 11 21 71.4%
C57BL/6NRj, C57BL/6NTac 2 6 15 53.3%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JRj 2 3 5 100.0%
C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 0 5 10 50.0%
C57BL/6J 2 0 6 33.3%
129X1/SvJ 0 2 38 5.3%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 0 2 2 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 1 0 3 33.3%
DBA/2NTac 1 0 48 2.1%
129S1/SvImJ 0 1 99 1.0%
C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 0 1 2 50.0%
C57BL/6J, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 0 1 1 100.0%
C57BL/6NRj 0 1 10 10.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: 129S1/SvImJ and 129X1/SvJ and C57BL/6J and C57BL/6NRj and DBA/2NTac
    • C57BL/6J: 132 / 176 (75.0%)
    • C57BL/6NRj: 15 / 38 (39.5%)
    • 129X1/SvJ: 2 / 38 (5.3%)
    • DBA/2NTac: 1 / 48 (2.1%)
    • 129S1/SvImJ: 1 / 99 (1.0%)
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two XY samples VM8959 and JA1465 at the time of importation and the conclusions are consistent in both samples. The results of the analysis are congruent with the SDS and VM8959 is the representative sample selected for the report.

This sample has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect. None of the contructs detectable by MiniMUGA are present. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.
Special Considerations Based on this report, a request has been made to update the strain name from B6.Cg-Get3tm1Hbha/Mmnc to B6;129(Cg)-Get3tm1Hbha/Mmnc. (April 11, 2023).
WARNINGS
  • There is a discrepancy between the diagnostic backgrounds detected ((129S1/SvImJ and 129X1/SvJ and C57BL/6J and C57BL/6NRj and DBA/2NTac)) and the primary background (C57BL/6J and C57BL/6NTac) and secondary background (129X1/SvJ). This is uncommon and should be investigated further.
  • The presence of a single diagnostic heterozygous call for a single inbred strain should be treated with caution.
  • This sample likely has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect. This can potentially be addressed with input from the user.
Diplotype Intervals
Report generated on 2023-04-11 18:39