MiniMUGA Sample Report - Initial Version |
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| MMRRC Strain Name | B6;129S4-Myh9tm3(GFP/MYH9/MYH10)Rsad/Mmnc | |||||||||||||||||||||||||||||||||||||||||||||
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| MMRRC Strain ID | 34322 | |||||||||||||||||||||||||||||||||||||||||||||
| Sample ID | VM4183 | |||||||||||||||||||||||||||||||||||||||||||||
| Genotyping Quality |
Excellent (0 N calls)
All reported results are dependent on genotyping quality. |
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| Chromosomal Sex | XY | |||||||||||||||||||||||||||||||||||||||||||||
| Genome Analysis |
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| Constructs Detected |
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Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
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| Strain Reproducibility |
Strain Reproducibility Samples: VM4183, SQ3576
Inbreeding Level: 98.2% Y Chromosome: Host Congenicity Level: 89.9% Donor Contribution in Linked Interval: 62.4Mb The genome of this strain is not reproducible because there are regions that are still segregating (at least 1.8%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown. |
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| Notes | Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on two samples VM41833 (XY), SQ3576 (XX) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are fully congruent with the SDS and VM41833 is the representative sample selected for the report. The primary (host) background is C57BL/6. The secondary (donor) background is derived from an F1 background (B6/129S4) which is consistent with the original nomenclature in the SDS and the background of the targeted allele could be either B6 or S4. Inspection of the ideogram from both samples supports the idea that targeted allele is on an 129S4 background. This can be confirmed by survival analysis in the progeny of het X het crosses. The mitochondrial genome is consistent with at least one backcross to a female from the host background. The Y chromosome is consistent with at least one backcross to a male from the host background. Both samples are heterozygous for the donor background at the locus. The following construct(s) are detected in all samples: bpA, GFP. The genome of the MMRRC:34322 strain is 98.2 % inbred and is 89.9% congenic at the strain level. The donor contribution in the linked interval spans 62.4Mb. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible. |
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| Diplotype Intervals | ||||||||||||||||||||||||||||||||||||||||||||||