MiniMUGA Sample Report - Initial Version
MMRRC Strain Name C57BL/6N-Col1a2tm1b(EUCOMM)Wtsi/LaelMmnc
MMRRC Strain ID 66964
Sample ID SN3483
Genotyping Quality Excellent (8 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2022-10-02T21:53:24.426277 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
C57BL/6NTac Homozygous 9225 100.0% 100.0%
Total 9225 100.0% 100.0%
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - - - - - - + - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6NCrl, C57BL/6NRj, C57BL/6NTac 10 1 13 84.6%
C57BL/6NCrl, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 6 1 7 100.0%
C57BL/6NJ 5 3 42 19.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl 2 2 20 20.0%
C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 3 0 3 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 2 0 2 100.0%
C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 1 2 3 100.0%
C57BL/6NRj, C57BL/6NTac 1 1 2 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl, C57BL/6NRj 0 1 10 10.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: C57BL/6NCrl and C57BL/6NTac and C57BL/6NJ
    • C57BL/6NCrl or C57BL/6NTac: 11 / 13 (84.6%)
    • C57BL/6NCrl or C57BL/6NJ or C57BL/6NTac: 12 / 12 (100.0%)
    • C57BL/6NJ: 8 / 42 (19.0%)
    • C57BL/6NCrl: 5 / 30 (16.7%)
    • C57BL/6NJ or C57BL/6NTac: 3 / 3 (100.0%)
    • C57BL/6NTac: 2 / 2 (100.0%)
  • Solution 2: C57BL/6NTac and C57BL/6NJ and B6N-Tyr/BrdCrCrl
    • C57BL/6NTac: 13 / 15 (86.7%)
    • C57BL/6NJ or C57BL/6NTac: 13 / 13 (100.0%)
    • C57BL/6NJ: 8 / 42 (19.0%)
    • B6N-Tyr/BrdCrCrl: 5 / 30 (16.7%)
    • B6N-Tyr/BrdCrCrl or C57BL/6NJ or C57BL/6NTac: 2 / 2 (100.0%)
  • Solution 3: C57BL/6NCrl and C57BL/6NRj and C57BL/6NJ
    • C57BL/6NCrl or C57BL/6NRj: 12 / 23 (52.2%)
    • C57BL/6NCrl or C57BL/6NJ or C57BL/6NRj: 12 / 12 (100.0%)
    • C57BL/6NJ: 8 / 42 (19.0%)
    • C57BL/6NCrl: 4 / 20 (20.0%)
    • C57BL/6NJ or C57BL/6NRj: 3 / 3 (100.0%)
    • C57BL/6NRj: 2 / 2 (100.0%)
  • Solution 4: C57BL/6NRj and C57BL/6NJ and B6N-Tyr/BrdCrCrl
    • C57BL/6NRj: 13 / 15 (86.7%)
    • C57BL/6NJ or C57BL/6NRj: 13 / 13 (100.0%)
    • C57BL/6NJ: 8 / 42 (19.0%)
    • B6N-Tyr/BrdCrCrl: 4 / 20 (20.0%)
    • B6N-Tyr/BrdCrCrl or C57BL/6NJ or C57BL/6NRj: 2 / 2 (100.0%)
    • B6N-Tyr/BrdCrCrl or C57BL/6NRj: 1 / 10 (10.0%)
Strain Reproducibility Strain Reproducibility Samples: SN3483, AA6555
Inbreeding Level: 100.0%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 0.0Mb
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two XY samples, AA6555 and SN3483 , at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and SN3483 is the representative sample selected for the report. The strain background is C57BL/6N. The mitochondrial genome and Y chromosome are consistent with the C57BL/6N background. The strain is 100 % inbred. The following construct(s) are detected in all samples: IRES
Diplotype Intervals
Report generated on 2022-10-02 21:53