MiniMUGA Sample Report - Initial Version
MMRRC Strain Name STOCK Smarca4tm1.2Pcn/Mmnc
MMRRC Strain ID 36548
Sample ID SB9519
Genotyping Quality Excellent (9 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XX
2023-02-28T16:08:17.182512 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
C57BL/6J and C57BL/6NTac Homozygous 1658 51.5% 51.6%
C3H/HeNHsd Homozygous 768 23.8% 24.0%
C57BL/6J and C57BL/6NTac X C3H/HeNHsd Heterozygous 529 16.4% 15.8%
Unexplained Homozygous 267 8.3% 8.5%
Total 3222 100.0% 100.0%
* Please see substrain reproducibility section
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - + - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6J, C57BL/6JRj 34 19 139 38.1%
C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 8 0 21 38.1%
C57BL/6NRj, C57BL/6NTac 2 0 15 13.3%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 1 0 2 50.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JRj 0 2 5 40.0%
C3H/HeNCrl, C3H/HeNHsd, C3H/HeNRj, C3H/HeNTac 1 0 5 20.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 0 1 1 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 0 1 3 33.3%
C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 0 1 10 10.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: C3H/HeNCrl and C57BL/6J and C57BL/6NTac
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNCrl: 1 / 5 (20.0%)
  • Solution 2: C3H/HeNHsd and C57BL/6J and C57BL/6NTac
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNHsd: 1 / 5 (20.0%)
  • Solution 3: C3H/HeNTac and C57BL/6J and C57BL/6NTac
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNTac: 1 / 5 (20.0%)
  • Solution 4: C3H/HeNCrl and C57BL/6JRj and C57BL/6NTac
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNCrl: 1 / 5 (20.0%)
  • Solution 5: C3H/HeNCrl and C57BL/6J and C57BL/6NRj
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNCrl: 1 / 5 (20.0%)
  • Solution 6: C3H/HeNRj and C57BL/6J and C57BL/6NTac
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNRj: 1 / 5 (20.0%)
  • Solution 7: C3H/HeNHsd and C57BL/6JRj and C57BL/6NTac
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNHsd: 1 / 5 (20.0%)
  • Solution 8: C3H/HeNTac and C57BL/6JRj and C57BL/6NTac
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNTac: 1 / 5 (20.0%)
  • Solution 9: C3H/HeNHsd and C57BL/6J and C57BL/6NRj
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNHsd: 1 / 5 (20.0%)
  • Solution 10: C3H/HeNTac and C57BL/6J and C57BL/6NRj
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNTac: 1 / 5 (20.0%)
  • Solution 11: C3H/HeNCrl and C57BL/6JRj and C57BL/6NRj
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNCrl: 1 / 5 (20.0%)
  • Solution 12: C3H/HeNRj and C57BL/6JRj and C57BL/6NTac
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NTac: 4 / 28 (14.3%)
    • C3H/HeNRj: 1 / 5 (20.0%)
  • Solution 13: C3H/HeNRj and C57BL/6J and C57BL/6NRj
    • C57BL/6J: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNRj: 1 / 5 (20.0%)
  • Solution 14: C3H/HeNHsd and C57BL/6JRj and C57BL/6NRj
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNHsd: 1 / 5 (20.0%)
  • Solution 15: C3H/HeNTac and C57BL/6JRj and C57BL/6NRj
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNTac: 1 / 5 (20.0%)
  • Solution 16: C3H/HeNRj and C57BL/6JRj and C57BL/6NRj
    • C57BL/6JRj: 65 / 168 (38.7%)
    • C57BL/6NRj: 4 / 28 (14.3%)
    • C3H/HeNRj: 1 / 5 (20.0%)
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two samples, SB9519 (XX ), and CU0798 (XY), at the time of importation. SB9519 is the representative sample selected for the report. The primary (host) background is C57BL/6. The g_FP construct is detected in the sample.

This sample has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect.

The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.

Special Considerations Based on this report, a request has been made to update the strain name from B6;129S2-Smarca4tm1.2Pcn/Mmnc to STOCK Smarca4tm1.2Pcn/Mmnc (February 4, 2023).

MiniMUGA has detected a potential unexpected construct (g_FP) that may interfere with the experimental design/rigor and reproducibility. It is recommended to genotype to ensure that g_FP is not present.
WARNINGS
  • This sample likely has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect. This can potentially be addressed with input from the user.
Diplotype Intervals
Report generated on 2023-02-28 16:08