MiniMUGA Sample Report - Initial Version |
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| MMRRC Strain Name | B6N(Cg)-Dsppem2Jpsi/Mmnc | ||||||||||||||||||||||||||||||||||||||
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| MMRRC Strain ID | 67180 | ||||||||||||||||||||||||||||||||||||||
| Sample ID | RR9773 | ||||||||||||||||||||||||||||||||||||||
| Genotyping Quality |
Excellent (2 N calls)
All reported results are dependent on genotyping quality. |
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| Chromosomal Sex | XY | ||||||||||||||||||||||||||||||||||||||
| Genome Analysis |
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| Constructs Detected |
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Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
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| Strain Reproducibility |
Strain Reproducibility Samples: RR9773, PC9140
Inbreeding Level: 100.0% Y Chromosome: Host Congenicity Level: 100.0% Donor Contribution in Linked Interval: 0.0Mb |
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| Notes | Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on two samples RR9773 (XY) and PC9140 (XX), at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and RR9773 is the representative sample selected for the report. The primary background is C57BL/6NCrl. This is a line that was generated by CRISPR genome editing in a C57BL/6N X SJL F1 background and no SJL contribution is detected by MiniMUGA. The mitochondrial genome is consistent with at least one backcross to a female from the host background. The Y chromosome is consistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected C57BL/6NCrl background . Both samples are homozygous for the C57BL/6NCrl background at the locus and none of the constructs detectable by MiniMUGA are present. The genome of the MMRRC strain: 67180 is 100 % inbred at the strain level. The level of congenicity estimated by MiniMUGA is greater than what would be predicted from the number of backcross generations reported in the SDS (2). Special Considerations: Based on this report, a request has been made to update the strain name from B6N;SJL-Dsppem2Jpsi/Mmnc to B6N(Cg)-Dsppem2Jpsi/Mmnc (1/3/2023) |
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| Diplotype Intervals | |||||||||||||||||||||||||||||||||||||||