Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on one XY samples (QZ1372) at the time of importation and the results of the analysis are congruent with the SDS. The background is C57BL/6NTac and there is no evidence of a secondary background. The mitochondrial genome and Y chromosome are consistent with C57BL/6NTac. The Cre construct is detected in the sample.
The genome of the MMRRC: 37396 strain is 100 % inbred and 100% congenic at the strain/substrain level which is consistent with the number of backcross generations reported in the SDS.
WARNINGS
There is a discrepancy between the diagnostic backgrounds detected ((C57BL/6J and C57BL/6NRj) or (C57BL/6JRj and C57BL/6NRj)) and the primary background (C57BL/6NTac) and secondary background (C57BL/6J). This is uncommon and should be investigated further.