MiniMUGA Sample Report - Initial Version |
|||||||||||||||||||||||||||||||||||||||||||||||||||
| MMRRC Strain Name | B6.129X1-Gt(ROSA)26Sortm2(ECFP)Cos/Mmnc | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MMRRC Strain ID | 15748 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample ID | NR3313 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Genotyping Quality |
Excellent (2 N calls)
All reported results are dependent on genotyping quality. |
||||||||||||||||||||||||||||||||||||||||||||||||||
| Chromosomal Sex | XY | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Genome Analysis |
|
||||||||||||||||||||||||||||||||||||||||||||||||||
| Constructs Detected |
|
||||||||||||||||||||||||||||||||||||||||||||||||||
|
Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
|
||||||||||||||||||||||||||||||||||||||||||||||||||
| Strain Reproducibility |
Strain Reproducibility Samples: UY8135, NR3313
Inbreeding Level: 96.6% Y Chromosome: Donor Congenicity Level: 99.2% Donor Contribution in Linked Interval: 118.9Mb The genome of this strain is not reproducible because there are regions that are still segregating (at least 3.4%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown. |
||||||||||||||||||||||||||||||||||||||||||||||||||
| Substrain Reproducibility |
89.8% of the genome is distinguishable between two substrains contributing to the primary background. Of this:
Ideogram 1 Ideogram 2 |
||||||||||||||||||||||||||||||||||||||||||||||||||
| Notes |
Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on two XY sample,s NR3313 and UY8135, at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and NR3313 is the representative sample selected for the report. The primary (host) background is C57BL/6J. The secondary (donor) background is consistent with 129X1/SvJ, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with both the host and donor background (identical by descent). The Y chromosome is inconsistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected donor background (129X1/SvJ). Both sample are homozygous for the donor background at the locus. The following constructs are detected in all samples: bpA and g_FP. g_FP probes detect the expected CFP construct. The genome of MMRRC: 015748 mice strain is 96.6 % inbred and 99.2% congenic at the strain level. 3.4% of the genome is segregating for the secondary (donor) background. The level of congenicity estimated by miniMUGA is consistent with the number of backcross generations reported in the SDS. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible. |
||||||||||||||||||||||||||||||||||||||||||||||||||
| Special Considerations | The Y chromosome genome does not match the host (primary) background. | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Diplotype Intervals | |||||||||||||||||||||||||||||||||||||||||||||||||||