MiniMUGA Sample Report - Initial Version |
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| MMRRC Strain Name | C57BL/6-Rgs12tm1.1Dski/Mmnc | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| MMRRC Strain ID | 46281 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample ID | MA1094 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Genotyping Quality |
Excellent (1 N calls)
All reported results are dependent on genotyping quality. |
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| Chromosomal Sex | XY | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Genome Analysis |
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| Constructs Detected |
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Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
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| Strain Reproducibility |
Strain Reproducibility Samples: MA1094, WU0180
Inbreeding Level: 100.0% Y Chromosome: Host Congenicity Level: 100.0% Donor Contribution in Linked Interval: 0.0Mb |
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| Substrain Reproducibility |
100.0% of the genome is distinguishable between two substrains contributing to the primary background. Of this:
Ideogram 1 Ideogram 2 |
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| Notes | Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on two samples MA1094 (XY), WU0180 (XX) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and MA1094 is the representative sample selected for the report. The primary (host) background is C57BL/6 and there is positive evidence of the, C57BL/6J and C57BL/6N substrains. The mitochondrial genome is consistent with the C57BL/6 background and the Y chromosome is consistent with at least one backcross to C57BL/6J. Both samples are homozygous for the C57BL/6N background at the locus. The following construct(s) is/are detected in all samples: bpA, Flp, hCMV_a, hCMV_b, IRES. The genome of MMRRC:046281 mice is 83.9% inbred at the substrain level 16.1% of the genome is segregating for the two substrains,C57BL/6J and C57BL/6N. The contribution of C57BL/6J is 69.8%. The contribution of C57BL/6N is 30.2%. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible. |
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| Special Considerations | MiniMUGA has detected an unexpected construct (flp) that may interfere with the experimental design/rigor and reproducibility.
Based on this report, a request has been made to update the strain name from C57BL/6J-Rgs12tm1.1Dski/Mmnc to C57BL/6-Rgs12tm1.1Dski/Mmnc. |
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| Diplotype Intervals | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||