MiniMUGA Sample Report - Initial Version
MMRRC Strain Name C57BL/6J-Kif6em1(R556Vfs*7)Rgray/Mmnc
MMRRC Strain ID 50625
Sample ID IH7195
Genotyping Quality Excellent (1 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2022-10-02T21:56:52.185928 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers
Informative
Markers %
Genome %
C57BL/6J Homozygous 9215 100.0% 100.0%
Total 9215 100.0% 100.0%
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - - - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6J, C57BL/6JRj 132 1 142 93.7%
C57BL/6J, C57BL/6JEiJ, C57BL/6JRj 21 0 21 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JRj 5 0 5 100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj 2 0 2 100.0%
C57BL/6J 0 4 4 100.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: C57BL/6J
    • C57BL/6J: 165 / 174 (94.8%)
Strain Reproducibility Strain Reproducibility Samples: IH7195, SM9995
Inbreeding Level: 100.0%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 0.0Mb
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two XY samples, SM9995 and IH7195, at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and IH7195 is the representative sample selected for the report. The strain background is C57BL/6J. The mitochondrial genome and Y chromosome are consistent with the C57BL/6J background and none of the constructs detectable by MiniMUGA are present.

The genome of the MMRRC:50625 strain is 100 % inbred.
Diplotype Intervals
Report generated on 2022-10-02 21:56