MiniMUGA Sample Report

MiniMUGA Sample Report - Initial Version

MMRRC Strain Name

B6J.129S6-Tph2^tm1Mca/McaMmnc

MMRRC Strain ID

43618

Sample ID

HX0878

Genotyping Quality

Excellent (12 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Genome Analysis

Background	Zygosity	Informative Markers	Informative Markers %	Genome %
C57BL/6J	Homozygous	2853	99.7%	99.3%
C57BL/6J X 129S6/SvEvTac	Heterozygous	10	0.3%	0.7%
Total		2863	100.0%	100.0%

Constructs Detected

BlastR	bpA	Cas9	chlor	cHS4	Cre	DTA	Flp	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Diagnostic Class	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J, C57BL/6JRj	128	5	142	93.7%
C57BL/6J, C57BL/6JEiJ, C57BL/6JRj	21	0	21	100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JRj	5	0	5	100.0%
C57BL/6J	3	0	4	75.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj	2	0	2	100.0%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6J, C57BL/6JEiJ, C57BL/6JRj	1	0	1	100.0%
C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj	1	0	1	100.0%
C57BL/6J, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRj	1	0	1	100.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):

Solution 1: C57BL/6J

C57BL/6J: 167 / 177 (94.4%)

Strain Reproducibility

Strain Reproducibility Samples: LX0733, HX0878
Inbreeding Level: 99.3%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 19.1Mb

The genome of this strain is not reproducible because there are regions that are still segregating (at least 0.7%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown.

Notes

Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two samples LX0733 (XX), HX0878 (XY) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and HX0878 is the representative sample selected for the report. The primary (host) background is C57BL/6J. The secondary (donor) background is consistent with 129S6/SvEvTac, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with at least one backcross to a female from the host background. The Y chromosome is consistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected donor background (129S6/SvEvTac). Both samples are heterozygous for the donor background at the locus. None of the constructs detectable by MiniMUGA are present.

The genome of MMRRC:043618 mice is 99.3 % inbred and 100% congenic at the strain level. 0.7% of the genome is segregating for the secondary (donor) background. The level of congenicity estimated by miniMUGA is consistent with the number of backcross generations reported in the SDS.