MiniMUGA Sample Report - New Algorithm
MMRRC Strain Name 129S.B6-Stub1tm1Cpat/Mmnc
MMRRC Strain ID 37469
Sample ID FR4869
Genotyping Quality Excellent (21 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2023-05-23T17:42:43.202417 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers
Informative
Markers %
Genome %
129S6/SvEvTac Homozygous 2700 99.9% 100.0%
C3H/HeJ Homozygous 1 0.0% 0.0%
129S6/SvEvTac X C3H/HeJ Heterozygous 2 0.1% 0.0%
Total 2703 100.0% 100.0%
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- + - - - - - - - - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
129S6/SvEvTac 39 0 40 97.5%
129S4/SvJaeJ, 129S6/SvEvTac 6 1 7 100.0%
129S1/SvImJ, 129S2/SvHsd, 129S2/SvPasOrlRj, 129S4/SvJaeJ, 129S6/SvEvTac 2 0 2 100.0%
129S1/SvImJ, 129S4/SvJaeJ, 129S6/SvEvTac 2 0 2 100.0%
C3H/HeJ 0 1 171 0.6%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: 129S6/SvEvTac and C3H/HeJ
    • 129S6/SvEvTac: 50 / 51 (98.0%)
    • C3H/HeJ: 1 / 171 (0.6%)
Strain Reproducibility Strain Reproducibility Samples: FR4869, AB6548, UP3063
Inbreeding Level: 100.0%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 0.0Mb
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two XY samples (FR4869, AB6548) and one XX sample (UP3063) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are fully congruent with the SDS and FR4869 is the representative sample selected for the report. The strain background is 129S6/SvEvTac and there is no evidence of a secondary background. The mitochondrial genome and Y chromosome are consistent with 129S6/SvEvTac. The genome of the MMRRC: 37469 strain is 100 % inbred. The following construct is detected in all samples: bpA.
WARNINGS
  • The presence of a single diagnostic heterozygous call for a single inbred strain should be treated with caution.
Diplotype Intervals
Report generated on 2023-05-23 17:42