MiniMUGA Sample Report - Initial Version |
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| MMRRC Strain Name | B6J.B6N-Ube3atm1a(KOMP)Wtsi/Mmnc | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| MMRRC Strain ID | 37375 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample ID | FL4354 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Genotyping Quality |
Excellent (2 N calls)
All reported results are dependent on genotyping quality. |
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| Chromosomal Sex | XY | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Genome Analysis |
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| Constructs Detected |
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Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
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| Strain Reproducibility |
Strain Reproducibility Samples: LT5327, FL4354
Inbreeding Level: 100.0% Y Chromosome: Host Congenicity Level: 100.0% Donor Contribution in Linked Interval: 0.0Mb |
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| Substrain Reproducibility |
100.0% of the genome is distinguishable between two substrains contributing to the primary background. Of this:
Ideogram 1 Ideogram 2 |
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| Notes | I. Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on two XY samples (FL4354 and LT5327) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and FL4354 is the representative sample selected for the report. The background is C57BL/6. There is positive evidence of C57BL/6N and C57BL/6J substrains and there is no evidence of additional backgrounds. The mitochondrial genome is consistent with both substrain backgrounds (identical by descent). The Y chromosome is not consistent with at least one backcross to a male from the C57BL/6J background. The region containing the allele of interest has the expected background (C57BL/6N). Both samples are homozygous for theC57BL/6N background at the locus. None of the constructs detectable by MiniMUGA are present. The genome of the MMRRC: 37375 strain is 100 % inbred and 100% congenic at the strain level. 15.8% of the genome is segregating for the two substrains (C57BL/6J and C57BL/6N) that contribute to the background. The contribution of C57BL/6J is 86.5%. The contribution of C57BL/6N is 13.5%. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible. |
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| Special Considerations | Y chromosome does not match the C57BL/6J) background. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Diplotype Intervals | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||