MiniMUGA Sample Report - Initial Version
MMRRC Strain Name C57BL/6-Tg(APOE-SCARB1*)13Arem/Mmnc
MMRRC Strain ID 41572
Sample ID FG5358
Genotyping Quality Excellent (9 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2023-07-28T21:17:13.205319 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
C57BL/6J and C57BL/6NTac N/A 8162 100.0% 100.0%
Total 8162 100.0% 100.0%
* Please see substrain reproducibility section
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - - - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 10 0 10 100.0%
C57BL/6NRj, C57BL/6NTac 2 3 15 33.3%
B6N-Tyr<c-Brd>/BrdCrCrl, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 2 0 2 100.0%
C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 2 0 2 100.0%
C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac 1 0 1 100.0%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: C57BL/6NTac
    • C57BL/6NTac: 20 / 30 (66.7%)
  • Solution 2: C57BL/6NRj
    • C57BL/6NRj: 20 / 30 (66.7%)
Strain Reproducibility Strain Reproducibility Samples: FG5358
Inbreeding Level: 100.0%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 0.0Mb
Substrain Reproducibility 100.0% of the genome is distinguishable between two substrains contributing to the primary background. Of this:
  • Level of inbreeding: 98.9%
  • C57BL/6J Contributes: 0.5%
  • C57BL/6NTac Contributes: 99.5%
The genome of this strain is not reproducible because there are regions that are still segregating (1.1%) at the substrain level. The estimation of contribution of substrains depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown.
Ideogram 1
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on one XY samples (FG5358) at the time of importation and the results of the analysis are congruent with the SDS. The background is C57BL/6N and there is no evidence of a secondary background. The mitochondrial genome and Y chromosome are consistent with C57BL/6N. None of the constructs detectable by MiniMUGA are present.

The genome of the MMRRC: 41572 strain is 100 % inbred and 100% congenic at the strain/substrain level.
Diplotype Intervals
Report generated on 2023-07-28 21:17