MiniMUGA Sample Report - Initial Version
MMRRC Strain Name BALB/cCrlAlt.Cg-Cecr2tm1.1Hemc/Mmnc
MMRRC Strain ID 43818
Sample ID CY7776
Genotyping Quality Excellent (23 N calls)
All reported results are dependent on genotyping quality.
Chromosomal Sex XY
2023-03-02T21:19:03.476505 image/svg+xml Matplotlib v3.5.1, https://matplotlib.org/
Genome Analysis
Background Zygosity Informative
Markers		 Informative
Markers %		 Genome %
BALB/cAnNCrl Homozygous 2767 99.7% 99.7%
BALB/cAnNCrl X C57BL/6NTac Heterozygous 7 0.3% 0.3%
Total 2774 100.0% 100.0%
Constructs Detected
BlastR
bpA
Cas9
chlor
cHS4
Cre
DTA
Flp
g_FP
hCMV_a
hCMV_b
hTK_pr
iCre
IRES
Luc
r_FP
rtTA
SV40
tTA
- - - - - - - - - - - - - - - - - - -
Backgrounds Detected
(Diagnostic Alleles)
  Diagnostic Alleles Observed
Diagnostic Class Homozygous Heterozygous Potential % Observed
BALB/cAnNCrl, BALB/cAnNHsd, BALB/cAnNRj, BALB/cByJ, BALB/cByJRj 8 0 8 100.0%
BALB/cAnNCrl, BALB/cAnNHsd, BALB/cAnNRj 7 0 17 41.2%

Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
  • Solution 1: BALB/cAnNHsd
    • BALB/cAnNHsd: 15 / 25 (60.0%)
  • Solution 2: BALB/cAnNCrl
    • BALB/cAnNCrl: 15 / 25 (60.0%)
  • Solution 3: BALB/cAnNRj
    • BALB/cAnNRj: 15 / 25 (60.0%)
Strain Reproducibility Strain Reproducibility Samples: AE1055, CY7776
Inbreeding Level: 99.7%
Y Chromosome: Host
Congenicity Level: 100.0%
Donor Contribution in Linked Interval: 8.1Mb

The genome of this strain is not reproducible because there are regions that are still segregating (at least 0.3%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown.
Notes Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on two samples AE1055 (XX), CY7776 (XY) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are fully consistent with the SDS and CY7776 is the representative sample shown in the report. The primary (host) background is consistent with the BALB/c strain group. A BALB/cCrlAlt substrain reference is not available in miniMUGA and BALB/cAnNCrl is the closest reference strain. The secondary (donor) background is consistent with C57BL/6NTac, which is in agreement with the background of the ES cell line used- iTL BA1 (C57BL/6NTac x 129S6/SvEvTac)F1). The mitochondrial genome and the Y chromosome are consistent with both the host and donor background (identical by descent). The strain is 99.75 % inbred and 100 % congenic. The region containing the allele of interest has the expected donor background (C57BL/6NTac). None of the constructs detectable by MiniMUGA are present.

The genome of the MMRRC: 43818 strain is 99.75 % inbred and 100% congenic at the strain level. 0.25% of the genome is segregating for the secondary (donor) background. The level of congenicity estimated by miniMUGA is consistent with the number of backcross generations reported in the SDS (10 Generations).
Diplotype Intervals
Report generated on 2023-03-02 21:19