MiniMUGA Sample Report - new algorithm |
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MMRRC Strain Name | B6J.129P2-Lpltm1Mae/Mmnc | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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MMRRC Strain ID | 43619 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample ID | BN1426 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Genotyping Quality |
Excellent (1 N calls)
All reported results are dependent on genotyping quality. |
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Chromosomal Sex | XY | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Genome Analysis |
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Constructs Detected |
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Backgrounds Detected
(Diagnostic Alleles) |
Minimal Strain Sets Explaining All Diagnostic Classes (Number of Markers Explained):
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Strain Reproducibility |
Strain Reproducibility Samples: BN1426
Inbreeding Level: 99.6% Y Chromosome: Donor Congenicity Level: 100.0% Donor Contribution in Linked Interval: 10.6Mb The genome of this strain is not reproducible because there are regions that are still segregating (at least 0.4%) at the strain level. The estimation of contribution of primary and secondary background depends on the number of samples genotyped. The impact of this on the published phenotype(s) is unknown. |
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Substrain Reproducibility |
98.6% of the genome is distinguishable between two substrains contributing to the primary background. Of this:
Ideogram 1 |
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Notes | Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.
GQC analysis was done on one XY sample (BN1426) at the time of importation. The results of the analysis are congruent with the SDS. The primary (host) background is C57BL/6. There is positive evidence of C57BL/6J and C57BL/6N substrains. The secondary (donor) background is consistent with 129P2/OlaHsd, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with both the host and donor background (identical by descent). The Y chromosome is inconsistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected donor background (129P2/OlaHsd). The sample is heterozygous for the donor background at the locus. None of the constructs detectable by MiniMUGA are present. The genome of the MMRRC: 43619 strain is 99.6 % inbred and 100% congenic at the strain level. 0.4.% of the genome is segregating for the secondary (donor) background. The level of congenicity estimated by miniMUGA is consistent with the number of backcross generations reported in the SDS. 70.1% of the genome is segregating for the two substrains (C57BL/6J and C57BL/6N) that contribute to the primary (host) background. The contribution of C57BL/6J is 50%. The contribution of C57BL/6N is 50%. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible. |
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Special Considerations | The Y chromosome does not match the host (primary) background
Based on this report, a request has been made to update the strain name from B6J.129P2-Lpltm1Mae/Mmnc to B6.129P2-Lpltm1Mae/Mmnc (May 23, 2023). |
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Diplotype Intervals |